Clostridium perfringens food poisoning: An update on toxins, occurrence, diseases and detection techniques
نویسندگان
چکیده
Clostridium perfringens is an anaerobe, gram positive, sporogenic, non-motile and encapsulated bacillus. The organism ubiquitous nature i.e. presence in soil, dust the gastrointestinal tracts of animals man, gives ample opportunity to contaminate foods. can exist as a heat-resistant spore, so it may survive cooking grow large numbers if cooked food held between 4 0C 60 for extensive time period. produces 17 different types extracellular toxins, which forms enterotoxaemia domestic wild several corners world. Besides poisoning, also rise sporadic diarrhoea, antibiotic-associated gas gangrene necrotic enteritis humans. These diseases have high economic impact due loss productivity death animals. Today, various methods been developed viz. cultural method, bioassay, immunological assay molecular techniques detect, identify characterize organism. isolation believed be most authentic method qualitative quantitative detection estimation perfringens. Since no single media alone found best suitable enumerate true strains Therefore, multimedia study required show status foods compare efficacy identification. Among current technique, immunoassay are extensively used microbiology diagnostic purpose epidemiological toxigenic pathogen. powerful, specific sensitive.
منابع مشابه
Outbreak of Clostridium perfringens food poisoning.
An outbreak of diarrhoea occurred at a 647-bedded long-stay hospital from 11 to 14 June 1989. Fifty-eight elderly residents developed symptoms and there were two deaths. The organism was identified as Clostridium perfringens type A, serotype TW23. The source of the outbreak was found to be inadequately reheated minced beef served at lunchtime on 11 June. The reason why only 4 of the possible 16...
متن کاملClostridium Perfringens Toxins Involved in Mammalian Veterinary Diseases
Clostridium perfringens is a gram-positive anaerobic rod that is classified into 5 toxinotypes (A, B, C, D, and E) according to the production of 4 major toxins, namely alpha (CPA), beta (CPB), epsilon (ETX) and iota (ITX). However, this microorganism can produce up to 16 toxins in various combinations, including lethal toxins such as perfringolysin O (PFO), enterotoxin (CPE), and beta2 toxin (...
متن کاملDistribution of neuraminidase among food-poisoning strains of Clostridium perfringens.
A survey was made to determine the distribution of the enzyme neuraminidase among 76 strains of Clostridium perfringens. Representative strains from each toxigenic type (A to F) and atypical C. perfringens type A food-poisoning strains of both American and English (Hobbs types) origin were tested. Both the American food-poisoning and nonfood-poisoning associated cultures consisted of both neura...
متن کاملSynthetic DNA probes for detection of enterotoxigenic Clostridium perfringens strains isolated from outbreaks of food poisoning.
Four synthetic oligonucleotides encoding different parts of the Clostridium perfringens enterotoxin gene were used to test the enterotoxigenicity of C. perfringens strains isolated from confirmed outbreaks of food poisoning. Of the 245 strains isolated from food and feces originating from 186 separate outbreaks, 145 (59%) gave hybridization reactions with each of the four DNA probes used, while...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Veterinary Sciences and Animal Husbandry
سال: 2023
ISSN: ['2456-2912']
DOI: https://doi.org/10.22271/veterinary.2023.v8.i3b.530